1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazines

ABSTRACT

Novel 1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazines, processes for the preparation thereof, and methods of treating psychoses, alleviating pain, and reducing blood pressure, employing compounds and compositions thereof are disclosed.

This is a division, of application Ser. No. 366,248 filed Apr. 9, 1982now U.S. Pat. No. 4,536,578.

DESCRIPTION OF THE INVENTION

The present invention relates to novel1-[(1,2-benzisoxazol-3-yl)propyl]piperazines. More particularly, thepresent invention relates to1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazinesof formula 1 ##STR1## wherein R is loweralkyl, benzyl, 2-hydroxyethyl,pyridyl or a group of the formula ##STR2## wherein R₁ is hydrogen,loweralkyl, loweralkoxy, halogen or trifluoromethyl; or pharmaceuticallyacceptable acid addition salts thereof, which are useful for treatingpsychoses, alleviating pain, and reducing blood pressure, alone or incombination with inert psychoses treating, pain alleviating, and bloodpressure reducing adjuvants.

Preferred1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazinesof forumula 1 are those wherein R is a group of the formula ##STR3##wherein R₁ is hydrogen, loweralkyl, loweralkoxy, halogen ortrifluoromethyl. More preferred1-[3-(6-fluoro-1,2-benzisoxazole-3-yl)propyl]-4-(substituted)piperazines are those wherein R₁ is loweralkoxy. Most preferred1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazinesare those wherein the loweralkoxy group is bound to the 2-position ofthe benzene ring.

As used through the specification and appended claims, the term "alkyl"refers to a straight or branched chain hydrocarbon radical containing nounsaturation and having 1 to 7 carbon atoms such as methyl, ethyl,1-propyl, 2-propyl, 1-butyl, 1-pentyl, 2-pentyl, 3-hexyl, 4-heptyl andthe like; the term "alkoxy" refers to a monovalent substituent whichconsists of an alkyl group linked through an ether oxygen and having itsfree valence bond from the ether oxygen such as methoxy, ethoxy,propoxy, butoxy, 1,1-dimethylethoxy, pentoxy, 3-methylpentoxy,2-ethylpentoxy and the like; the term "halogen" refers to a member ofthe family consisting of chlorine, fluorine, bromine or iodine. The term"lower" as applied to any of the aforementioned groups refers to a grouphaving a carbon skeleton containing up to and including 5 carbon atoms.

The compounds of the present invention which lack an element of symmetryexist as optical antipodes and as the racemic forms thereof. The opticalantipode may be prepared from the corresponding racemic forms bystandard optical resolution techniques, involving, for example, theseparation of diastereomeric salts of those instant compoundscharacterized by the presence of a basic amino group and an opticallyactive acid, or by the synthesis from optically active precursors.

The present invention comprehends all optical isomers and racemic formsthereof. The formulas of the compounds shown herein are intended toencompass all possible optical isomers of the compounds so depicted.

The novel1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(substituted)piperazinesof formula 1, the compounds of the present invention, are prepared bycondensing 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole of formula 2##STR4## the synthesis of which is described in U.S. patent applicationSer. No. 257,698, filed Apr. 27, 1981, with readily availablepiperazines of formula 3 ##STR5## wherein R is loweralkyl, benzyl,2-hydroxyethyl, pyridyl or a group of the formula ##STR6## wherein R₁ ishydrogen, loweralkyl, loweralkoxy, halogen or trifluoromethyl. Thecondensation is conveniently performed by treating the halide 2 with thepiperazine 3 in the presence of an acid acceptor, a displacementpromoter and a suitable solvent. Among acid acceptors, there may bementioned alkali metal carbonates and alkali metal bicarbonates such as,for example, lithium carbonate, sodium carbonate and potassiumcarbonate, and lithium bicarbonate, sodium bicarbonate and potassiumbicarbonate. Potassium carbonate and sodium bicarbonate are preferred.Among displacement promoters, there may be mentioned alkali metalhalides such as, for example, sodium iodide and potassium iodide, andsodium bromide and potassium bromide. Potassium iodide is preferred.Among suitable solvents, there may be mentioned polar aprotic substancessuch as, for example, dimethylformamide, dimethylacetamide andhexamethylphosphoramide. Dimethylformamide is preferred. The temperatureat which the condensation is conducted is not narrowly critical. It isdesirable, however, to perform the condensation at a temperature withinthe range of about 50° C. to about 130° C. to assure a reasonable rateof conversion. A reaction temperature within the range of about 70° C.to 110° C. is preferred.

The 1-[(1,2 benzisoxazol-3-yl)propyl]piperazines of the presentinvention are useful as analgesic agents due to their ability toalleviate pain in mammals which is demonstrated in thephenyl-para-quinone writhing assay in mice, a standard assay foranalgesia [Proc. Soc. Exptl. Biol. Med., 95, 729 (1957)]. Presented inTable I is the analgesic effect of some of the compounds of theinvention, expressed as the subcutaneous dose at which 50% of thephenyl-para-quinone induced writhing is inhibited in the animals, i.e.,the ED₅₀.

                  TABLE 1                                                         ______________________________________                                                             Analgesic Activity                                       Compound             ED.sub.50 (mg/kg)                                        ______________________________________                                        1-[3-(6-fluoro-1,2-benzisoxazol-                                                                   5.2                                                      3-yl)propyl]-4-(2-methoxyphenyl)-                                             piperazine)dihydrochloride                                                    1-[3-(6-fluoro-1,2-benzisoxazol-                                                                   0.8                                                      3-yl)propyl]-4-(2-pyridyl)piperazine                                          dihydrochloride                                                               1-[3-(6-fluoro-1,2-benzisoxazol-                                                                   1.8                                                      3-yl)propyl]-4-phenylpiperazine                                               oxalate                                                                       propoxyphene         3.9                                                      pentazocine          1.3                                                      ______________________________________                                    

Analgesia production is achieved when the present1-[(1,2-benzisoxazol-3-yl)propyl]piperazines are administered to subjectrequiring such treatment as an effective oral, parenteral or intravenousdose of from 0.1 to 50 mg/kg of body weight per day. A particularlyeffective amount is about 10 mg/kg of body weight per day. It is to beunderstood, however, that for any particular subject, specific dosageregimens should be adjusted according to the individual need and theprofessional judgment of the person administering or supervising theadministration of the aforesaid compound. It is to be further understoodthat the dosages set forth herein are exemplary only and that they donot, to any extent, limit the scope or practice of the invention.

The 1-[(1,2-benzisoxazol-3-yl)propyl]piperazines of the presentinvention are also useful as antihypertensives due to their ability toreduce blood pressure in mammals. Antihypertensive activity is measuredin the spontaneous hypertensive rat by the indirect tail cuff methoddescribed by A. Schwartz, Ed., "Methods in Pharmacology," Vol. 1,Appleton-Century-Crofts, New York, N.Y., 1971, page 135. According tothis procedure, the test compound is administered intraperitoneally to agroup of 4 rats for 3 days. The decrease in blood pressure is measuredon the third day of administration. The antihypertensive activity,expressed as the decrease in mean arterial blood pressure (mm ofmercury) in this procedure of some of the compounds of the presentinvention is presented in Table II.

                  TABLE II                                                        ______________________________________                                                         Dose      Blood Pressure                                                      (mg/kg of Decrease                                           Compound         Body wt)  (mm/mercury)                                       ______________________________________                                        1-[3-(6-fluoro-1,2-                                                                            50        66                                                 benzisoxazol-3-yl)-                                                           propyl]-4-(2-pyridyl)-                                                        piperazine dihydrochloride                                                    1-[3-(6-fluoro-1,2-                                                                            50        38                                                 benzisoxazol-3-yl)-                                                           propyl]-4-phenylpiperazine                                                    oxalate                                                                       1-[3-(6-fluoro-1,2-                                                                            50        66                                                 benzisoxazol-3-yl)-propyl]-                                                   4-(3-trifluoromethylphenyl)-                                                  piperazine hydrochloride                                                      4-(2-chlorophenyl)-1-                                                                          50        55                                                 [3-(6-fluoro-1,2-                                                             benzisoxazol-3-yl)-propyl]                                                    piperazine hydrochloride                                                      1-[3-(6-fluoro-1,2-                                                                            50        33                                                 benzisoxazol-3-yl)-propyl]-                                                   4-(2-methylphenyl)                                                            piperazine oxalate                                                            1-[3-(6-fluoro-1,2-                                                                            50        33                                                 benzisoxazol-3-yl)-propyl]-                                                   4-(4-fluorophenyl)                                                            piperazine oxalate                                                            guanethidine     50        20                                                 ______________________________________                                    

Blood pressure reduction is achieved when the present1-[(1,2-benzisoxazol-3-yl)propyl]piperazines are administered to asubject requiring such treatment as an effective oral, parenteral orintravenous dose of from 5.0 to 50 mg/kg of body weight per day. Aparticularly preferred effective amount is about 25 mg/kg of body weightper day. It is to be understood, however, that for any particularsubject, specific dosage regimens should be adjusted according to theindividual need and the professional judgment of the personadministering or supervising the administration of the aforesaidcompound. It is to be further understood that the dosages set forthherein are exemplary only and that they do not, to any extent, limit thescope or practice of the invention.

The 1-[(1,2-benzisoxazol-3-yl)propyl]piperazines of the presentinvention are useful for treating psychoses by virtue of their abilityto block apomorphine-induced climbing in mammals.

Antipsychotic activity is determined in the climbing mice assay by amethod similar to those described by P. Protais, et al.Psychopharmacol., 50, 1 (1976) and B. Costall, Eur. J. Pharmacol., 50,39 (1978).

The subject CK-1 male mice (23-27 grams) are group-housed under standardlaboratory conditions. The mice are individually placed in wire meshstick cages (4"×4" by 10") and are allowed one hour for adaptation andexploration of the new environment. Then apomorphine is injectedsubcutaneously at 1.5 mg/kg, a dose causing climbing in all subjects for30 minutes. Compounds to be tested for antipsychotic activity areinjected intraperitoneally 30 minutes prior to the apomorphine challengeat a screening dose of 10 mg/kg.

For evaluation of climbing, 3 readings are taken at 10, 20 and 30minutes after apomorphine administration according to the followingscale:

    ______________________________________                                        Climbing Behavior     Score                                                   ______________________________________                                        Mice With:                                                                    4 paws on bottom (no climbing)                                                                      0                                                       2 paws on the wall (rearing)                                                                        1                                                       4 paws on the wall (full climb)                                                                     2                                                       ______________________________________                                    

Mice consistently climbing before the injection of apormorphine will bediscarded.

With full-developed apomorphine climbing, the animals are hanging ontothe cage walls, rather motionless, over longer periods of time. Bycontrast, climbs due to mere motor stimulation usually only last a fewseconds.

The climbing scores are individually totaled (maximal score: 6 per mouseover 3 readings) and the total score of the control group (vehicleintraperitoneally--apomorphine subcutaneously) is set to 100%. ED₅₀values with 95% confidence limits are calculated by a Linear RegressionAnalysis. Antipsychotic activity expressed as the percentage decrease inclimbing score of some of the instant1-[(1,2-benzisoxazol-3-yl)propyl]piperazines as well as standardantipsychotics are presented in Table III.

                  TABLE III                                                       ______________________________________                                                                  Antipsychotic Activity                                              (mg/kg of (% decrease in                                      Compound        body wt)  climbing score)                                     ______________________________________                                        1-[3-(6-fluoro-1,2-benz-                                                                      4.1       50                                                  isoxazol-3-yl)propyl]-4-                                                      (2-methoxyphenyl)piperazine                                                   dihydrochloride                                                               1-[3-(6-fluoro-1,2-benz-                                                                      10        31                                                  isoxazol-3-yl)propyl]-4-                                                      (2-pyridyl)piperazine                                                         dihydrochloride                                                               1-[3-(6-fluoro-1,2-benz-                                                                      10        31                                                  isoxazol-3-yl)propyl]-4-                                                      phenylpiperazine oxalate                                                      haloperidol (standard)                                                                        0.11      50                                                  thioridazine (standard)                                                                       3.5       50                                                  ______________________________________                                    

Antipsychotic activity of the1-[(1,2-benzisoxazol-3-yl)propyl]piperazines of the present invention isalso demonstrated by their ability to block conditioned avoidance inmammals in the Sidman Avoidance Paradigm by a method similar to thatdescribed by M. Sidman, Science 118, 157 (1953).

The subjects are male squirrel monkeys (Saimiri sciureus). are housed inindividual home cages in the animal quarters for at least six weeksprior to training. When they are not in the test chambers, the subjectsare maintained in their home cages with water available ad libitum andfood available twice daily.

The apparatus consists of test cages (BRS/LVE) with a single lever andhouse-light. This is enclosed in a sound-attenuating chamber (BRS/LVE)with fan and the chambers are in an isolated room equipped with aspeaker emitting a white-noise auditory background. The test cages havea grid floor of steel bars which are attached to a scrambled shocksource (Coulbourn Instruments). The house-lights, shockers and responselevers are controlled by BRS/LVE electromechanical timer and controls.The records of responses and shocks are recorded on Coulbourn print-outcounters and Gerbrands cumulative recorders.

The monkeys are trained to avoid an unsignaled shock by repetitivelever-pressing responses. A shock (1.0 mA for 0.5 seconds) is deliveredto the grid floor every 20 seconds if no responses are made (shock-shockinterval of 20 seconds: SS-20"). A lever-press (response) will delay theoncoming shock for 20 seconds (Response-Shock interval of 20 seconds:RS-20"). The responses do not accumulate for delays of shock; a shockwill be delivered 20 seconds after the last response. Every 15 minutesthe total number of shocks received and the total number of responsesmade are accumulated and constitute the basic data. The animals aretrained until they maintain a stable response-rate and are receiving nomore than 50 shocks per 4 hour test session. After reaching thesecriteria of performance, experimental compounds are administered andtheir effects on the performance of this learned avoidance behavior areevaluated.

The drug's effect on performance of each animal is compared to theperformance data generated in the previous non-drug session. Each animalthereby serves as its own control. The basic measures of performanceduring a specific time interval, responses and shocks, are used forevaluation. Responses are reported both as totals and as percent ofcontrol responses. Shocks are reported as totals and as shock-avoided(SHA) as percent of control. This latter measure is computed bysubstracting the number of shocks received from the total number ofpossible shocks if no responses are made. For example, during a 15minute period, a total of 45 shocks are possible and if a subjectreceived 1 during the control session and 23 when treated with drug,=(45-1)/45×100=98% SHA in control and (45-23)/45×100=49% with drug and49/98×100=50% SHA as percent of control. Since the monkeys are all goodperformers and receive few shocks in non-drug conditions if the drugeffect on shock received were expressed as percent change in shocks, thereported results would be out of proportion to the actual effect. Theabove example would be expressed as a 2200% increase in shocks[(23-1)/1×100=2200%].

The experimental compounds are administered by oral intubationimmediately prior to testing in volumes of 1.0 cc/kg of body weight. Thecompounds are dissolved in distilled water or suspended with theaddition of one drop of Tween-80 per 10 cc of solution.

In the initial screening of experimental compounds the results arereported in terms of the total effect during a 4-hour test. However, anED₅₀ may be estimated during a representative time of peak activity.

Antipsychotic activity expressed as ED₅₀ -values of a representative1-[(1,2-benzisoxazol-3-yl)propyl]piperazine and two standards ispresented in Table IV.

                  TABLE IV                                                        ______________________________________                                                      Antipsychotic Activity                                                          Avoidance  Escape                                                             Response   Response                                                           (ED.sub.50 mg/kg                                                                         (ED.sub.50 mg/kg                                   Compound        of body wt)                                                                              of body wt)                                        ______________________________________                                        1-[3-(6-fluoro-1,2-                                                                           2.9        4.8                                                benzisoxazol-3-yl)-                                                           propyl]-4-(2-methoxy-                                                         phenyl)piperazine                                                             dihydrochloride                                                               haloperidol (standard)                                                                         1.21       1.27                                              thioridazine (standard)                                                                       2.6        3.5                                                ______________________________________                                    

The antipsychotic activity of the present1-[(1,2-benzisoxazol-3-yl)propyl]piperazines is enhanced as a result oftheir unexpectedly reduced propensity to cause undesirableextrapyramidal side effects (extrapyramidal symptomatology) in mammalsby a method similar to that described by J. Liebman and R. Neale,Psychopharmacology, 68, 25(1980).

Adult male squirrel monkeys (Saimiri sciurens) are housed in individualhome cages and fed twice daily with water available "ad libitum" whenthey are not in the test chambers.

The test monkeys have been "primed" with once-weekly oral doses ofhaloperidol until they repeatedly demonstrated dyskinetic reactions upondrug administration. At this point, the monkeys were included in thetest group and dosed orally with several standard drugs and experimentalcompounds to see if they elicited similar types of motor dysfunctions.

On the test day, drugs are administered by oral intubation immediatelyprior to testing in volumes of 1.0 cc/kg of body weight. The compoundsare dissolved in distilled water or suspended with the addition of onedrop of Tween-80 per 10 cc of solution.

After dosing, the monkeys are placed in observation chambers in anisolated room. Two experienced investigators observe the monkeys fordyskinetic symptomatology at 2, 4 and 6 hours postdose. They score themonkeys for the following specific dyskinetic symptomatology:

1. Circling--when the monkey rapidly circles the cage floor

2. "Duck walk"--when the monkey walks in a seated position with armscurled and held tightly to his side

3. Limb extension--an abnormal, prolonged extension of one or morelimbs, similar to but of longer duration than a stretching motion

4. Pushing--the monkey will push against the cage wall with his head orbody, frequently at the end of a circling episode

5. Writhing--slow, writhing movements of the whole body frequently inconjunction with a pushing episode

6. Oral dyskinesias--, chewing, gnawing and abnormal tongue protrusions

Normally, the monkey displays more than one type of dyskinesia during atest session but if any single dyskinesia is noted during an observationperiod, the monkey is given an overall positive E.P.S. score. Thefollowing scoring index is used in order to quantify the severity ofsymptoms:

    ______________________________________                                        SCORING INDEX FOR MONKEY EPS TEST                                             (DYSKINESIAS)                                                                 ______________________________________                                        CIRCLING:                                                                     1 incidence of 5 or more revolutions                                                   (under 30 sec.)                                                      2-4 incidences with one incidence over 30                                              seconds but under 1.5 minutes                                        5 or more incidences over 30 seconds each                                              or one incidence over 1.5 minutes                                    DUCK WALK:                                                                    one incidence under 5 seconds                                                 2-4 incidences each lasting between 5                                                  seconds and 1.5 minutes                                              5 incidences or 1 incidence lasting over                                               1.5 minutes                                                          LIMB EXTENSIONS:                                                              1-2 incidences under 10 seconds                                               3-5 incidences lasting between 10 seconds                                              and 2 minutes                                                        6 or more incidences or 1 incidence                                                    lasting over 2 minutes                                               PUSHING/WRITHING:                                                             1-2 incidences under 5 seconds each                                           3-5 incidences each lasting between 5                                                  seconds and 30 seconds                                               6 incidences or 1 incidence lasting over                                               30 seconds                                                           ______________________________________                                    

Extrapyramidal symptomatology of a representative1-[(1,2-benzisoxazol-3-yl)propyl]piperazine of the present invention andtwo standards is given in Table V.

                  TABLE V                                                         ______________________________________                                                        Dose      % Monkeys showing                                                   (mg/kg)   extrapyramidal                                      Compound        body wt)  symptoms                                            ______________________________________                                        1-[3-(6-fluoro-1,2-                                                                           3.5*       0                                                  benzisoxazol-3-yl)propyl]-                                                                    5.0        0                                                  4-(2-methoxyphenyl)-                                                                          10.0       66                                                 piperazine dihydrochloride                                                    haloperidol     0.625      33                                                                 1.3*      100                                                 thioridazine    3.5*      100                                                                 7.0       100                                                 ______________________________________                                         *ED.sub.50value in the Sidman Avoidance Paragidm in squirrel monkeys.    

Antipsychotic activity is achieved when the present1-[(1,2-benzisoxazol-3-yl)propyl]piperazines are administered to subjectrequiring such treatment as an effective oral, parenteral or intravenousdose of from 0.1 to 50 mg/kg of body per day. A particularly preferredeffective amount is about 5 mg/kg of body weight per day. It is to beunderstood, however, that for any particular subject, specific dosageregimens should be adjusted according to the individual need and theprofessional judgment of the person administering or supervising theadministration of the aforesaid compound. It is to be further understoodthat the dosages set forth herein are exemplary only and they do not, toany extent, limit the scope or practice of the invention.

Effective amounts of the compounds of the invention may be administeredto a subject by any one of various methods, for example, orally as incapsules or tablets, parenterally in the form of sterile solutions orsuspensions, and in some cases intravenously in the form of sterilesolutions. The free base final products, while effective themselves, maybe formulated and administered in the form of their pharmaceuticallyacceptable addition salts for purposes of stability, convenience orcrystallization, increased solubility and the like.

Preferred pharmaceutically acceptable addition salts include salts ofmineral acids, for example, hydrochloric acid, sulfuric acid, nitricacid and the like, salts of monobasic carboxylic acids such as, forexample, acetic acid, propionic acid and the like, salts of dibasiccarboxylic acids such as, for example, maleic acid, fumaric acid, oxalicacid and the like, and salts of tribasic cabocylic acids such as, forexample, carboxysuccinic acid, citric acid and the like.

The active compounds of the present invention may be administeredorally, for example, with an inert diluent or with an edible carrier.They may be enclosed in gelatin capsules or compressed into tablets. Forthe purpose of oral therapeutic administration, the aforesaid compoundsmay be incorporated with excipients and used in the form of tablets,troches, capsules, elixirs, suspensions, syrups, wafers, chewing gumsand the like. These preparations should contain at least 0.5% of activecompound, but may be varied depending upon the particular form and mayconveniently be between 4% to about 75% of the weight of the unit. Theamount of present compound in such composition is such that a suitabledosage will be obtained. Preferred compositions and preparationsaccording to the present invention are prepared so that an oral dosageunit form contains between 1.0-300 mgs of active compound.

The tablets, pills, capsules, troches and the like may also contain thefollowing ingredients: a binder such as microcrystalline cellulose, gumtragacanth or gelatin; an excipient such as starch or lactose, adisintegrating agent such as alginic acid, Primogel, corn starch and thelike; a lubricant such as magnesium stearate or Sterotes; a glidant suchas colloidal silicon dioxide; and a sweetening agent such as sucrose orsaccharin or a flavoring agent such as peppermint, methyl salicylate, ororange flavoring may be added. When the dosage unit form is a capsule,it may contain, in addition to materials of the above type, a liquidcarrier such as a fatty oil. Other dosage unit forms may contain othervarious materials which modify the physical form of the dosage unit, forexample, as coatings. Thus tablets or pills may be coated with sugar,shellac, or other enteric coating agents. A syrup may contain, inaddition to the active compounds, sucrose as a sweetening agent andcertain preservatives, dyes and colorings and flavors. Materials used inpreparing these various compositions should be pharmaceutically pure andnon-toxic in the amounts used.

For the purposes of parenteral therapeutic administration, the activecompounds of the invention may be incorporated into a solution orsuspension. These preparations should contain at least 0.1% of theaforesaid compound, but may be varied between 0.5 and about 50% of theweight thereof. The amount of active compound in such compositions issuch that a suitable dosage will be obtained. Preferred compositions andpreparations according to the present invention are prepared so that aparenteral dosage unit contains between 0.5 to 100 mgs of the activecompound.

The solutions or suspensions may also include the following components:a sterile diluent such as water for injection, saline solution, fixedoils, polyethylene glycols, glycerine, propylene glycol or othersynthetic solvents; antibacterial agents such as benzyl alcohol ormethyl parabens; antioxidants such as ascorbic acid or sodium bisulfite;chelating agents such as ethylenediaminetetraacetic acid; buffers suchas acetates, citrates or phosphates and agents for the adjustment oftonicity such as sodium chloride or dextrose. The parenteral preparationcan be enclosed in ampoules, disposable syringes or mutiple dose vialsmade of glass or plastic.

The following Examples are for illustrative purposes only and are not tobe construed as limiting the invention.

EXAMPLE 11-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-methoxyphenyl)piperazinedihydrochloride

To 30 ml of dry dimethylformamide was added 2.9 g of4-(2-methoxyphenylpiperazine), 3.4 g of3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 8.0 g of sodiumbicarbonate, and a few crystals of potassium iodide. The mixture washeated at 100° C. for two hrs, with stirring. The mixture was filteredand the filtrate was evaporated to an oil. The oil was stirred with 100ml of water for five mins, and extracted with ether. The ether extractwas washed with water (2×), saturated sodium chloride solution, anddried over anhydrous magnesium sulfate. After filtering, the solvent wasevaporated to an oil. The oil was dissolved in ether, and treated withethereal hydrogen chloride to give a salt. The salt was recrystallizedfrom ethyl acetate/methanol/ether to yield 2.8 g (42%) of product, mp208°-210° C. (dec).

ANALYSIS: Calculated for C₂₁ H₂₄ FN₃ O.2HCl: 57.02% C; 5.93% H; 9.50% N.Found: 57.11% C; 6.05% H; 9.33% N.

EXAMPLE 21-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-phenylpiperazine oxalate

To 35 ml of dry dimethylformamide was added 1.6 g of 4-phenylpiperazine,2.1 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 8.0 g of sodiumbicarbonate, and a few crystals of potassium iodide. The mixture washeated at 100° C. for three hrs, with stirring. The mixture was filteredand the filtrate was evaporated to an oil. The oil was stirred with 100ml of water for five mins and then extracted with ether. The etherextract was washed with water (2×), saturated sodium chloride solution,and dried over anhydrous magnesium sulfate. After filtering, thesolution was converted to 4.0 g (70%) of product, by treatment withethereal oxalic acid. Recrystallization twice from ethylacetate/methanol/ether yielded the analytical sample mp 188°-190° C.(dec).

ANALYSIS: Calculated for C₂₀ H₂₂ FN₃ O.(CO₂ H)₂ : 61.53% C; 5.63% H;9.79% N. Found: 61.14% C; 5.52% H; 9.85% N.

EXAMPLE 31-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(4-fluorophenyl)piperazineoxalate

A mixture of 5.0 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 4.0g of 1-(4-fluorophenyl)piperazine, 10 g of potassium carbonate and a fewcrystals potassium iodide in 70 ml of dimethylformamide was stirred at80° C. for 4.5 hr. The mixture was cooled, filtered and concentrated toan oil, which was stirred with water and extracted with ethylacetate-ether. The organic extracts were washed with water (2×),saturated sodium chloride solution, and dried over anhydrous magnesiumsulfate. The mixture was filtered and concentrated. The residue waspurified by column chromatography (silica gel, tetrahydrofuran) to givean oil. The oil was converted to 4.5 g (45%) of product by treatmentwith oxalic acid. Two recrystallizations from ethyl acetate/methanolgave the analytical sample, mp 186°-187° C. (dec).

ANALYSIS: Calculated for C₂₀ H₂₁ F₂ N₃ O.(CO₂ H)₂ : 59.05% C; 5.18% H;9.39% N. Found: 58.83% C; 5.15% H; 9.37% N.

EXAMPLE 44-(2-Chlorophenyl)-1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]piperazinehydrochloride

To 40 ml of dimethylformamide was added, 5.0 g of1-(2-chlorophenyl)piperazine hydrochloride hydrate, 6.4 g of3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10 g of milled potassiumcarbonate, and 0.01 g of potassium iodide, and the mixture was stirredat 90° C. for 2 hrs. The mixture was cooled, filtered and the filtrateevaporated to an oil. The oil was stirred with 100 ml water for fivemins and then extracted with ether. The ether extract was washed withwater (2×), saturated sodium chloride solution, and dried over anhydrousmagnesium sulfate. After filtering, the solution was acidified to pH 1with ethereal hydrogen chloride, and the precipitate was collected anddried to give 5.7 g (70%) of product, mp 195° C. (dec). Tworecrystallizations from ethyl acetate/methanol (5:1) gave the analyticalsample, mp 228° C. (dec).

ANALYSIS: Calculated for C₂₀ H₂₁ ClFN₃ O.HCl: 58.54% C; 5.41% H; 10.24%N; 17.28% Cl. Found: 58.58% C; 5.37% H; 10.13% N; 17.04% Cl.

EXAMPLE 51-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(3-trifluoromethylphenyl)piperazinehydrochloride

A mixture of 6.5 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 5.8g of N-(α,α,α-trifluoro-m-tolyl)piperazine, 7.0 g of potassium carbonateand a few crystals potassium iodide in 80 ml of dimethylformamide wasstirred at 70°-75° C. for seven hrs. The reaction mixture was cooled,filtered and concentrated to an oil, which was stirred with water andextracted with ether-ethyl acetate. The organic extracts were washedwith water (2×), saturated sodium chloride solution, and dried overanhydrous magnesium sulfate. The mixture was filtered and concentratedto an oil, which was converted to a salt by treatment with etherealhydrogen chloride. The salt was recystallized from ethylacetate/methanol to give 4 g (35%) of product mp, 208°-209° C.

ANALYSIS: Calculated for C₂₁ H₂₁ F₄ N₃ O.HCl: 56.82% C; 5.00% H; 9.47%N. Found: 56.63% C; 4.98% H; 9.4 % N.

EXAMPLE 61-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-methylphenylpiperazineoxalate

To 40 ml of dimethylformamide was added 5.0 g of4-(2-methylphenyl)piperazine dihydrochloride, 6.4 g of3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10.0 g of milledpotassium carbonate, and 0.01 g of potassium iodide. The mixture wasstirred at 90° C. for two hrs, cooled, filtered, then evaporated to anoil. The oil was stirred with 100 ml water for ten min and extractedwith ether. The ether extract was washed with water (2×), saturatedsodium chloride solution and dried over anhydrous magnesium sulfate.After filtering, ethereal oxalic acid was added and the resultingprecipitate was collected and dried to give 2.4 g (27%) of product. Tworecrystallizations from ethyl acetate/methanol/ether gave the analyticalsample, mp 177°-179° C.

ANALYSIS: Calculated for C₂₁ H₂₄ FN₃ O.(CO₂ H)₂ : 62.29% C; 5.91% H;9.48% N. Found: 62.03% C; 5.91% H; 9.36% N.

EXAMPLE 71-[3-(6-Flouro-1,2-benzisoxazol-3-yl)propyl]-4-(3-methoxyphenyl)piperazineoxalate

To 40 ml of dimethylformamide was added 5.3 g of4-(3-methoxyphenyl)piperazine dihydrochloride, 6.4 g of3-(3-chloropropvl)-6-fluoro-1,2-benzisoxazole, 10.0 g of milledpotassium carbonate, and 0.01 g of potassium iodide. The mixture wasstirred at 90° C. for two hrs, cooled, filtered, and evaporated to anoil. The oil was stirred with 100 ml water for ten mins, and extractedwith ether. The ether extract was washed with water (2×), saturatedsodium chloride solution and dried over anhydrous magnesium sulfate.After filtering, ethereal oxalic acid was added and the resultingprecipitate was collected and dried to give 3.8 g (41%) of product. Tworecrystallizations from ethyl acetate/methanol/ether (10:1:5), gave theanalytical sample, mp 168° C. (dec).

ANALYSIS: Calculated for C₂₁ H₂₄ FN₃ O₂.(CO₂ H)₂ : 60.12% C; 5.70% H;9.15% N. Found: 60.01% C; 5.71% H; 9.06% N.

EXAMPLE 81-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(4-methoxyphenyl)piperazineoxalate

To 40 ml of dimethylformamide was added 4.3 g3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 4.0 g of4-(4-methoxyphenyl)piperazine dihydrochloride, 10.0 g of milledpotassium carbonate, and 0.01 g potassium iodide. The mixture wasstirred at 90° C. for two hrs, cooled, filtered, and the filtrateevaporated to an oil. The oil was stirred with 100 ml of water andextracted with ether. The ether extract was washed with water (2×),saturated sodium chloride solution and dried over anhydrous magnesiumsulfate. After filtering, ethereal oxalic acid was added. Theprecipitate was collected and dried to yield 3.0 g (44%) of product. Tworecrystallizations from ethyl acetate/methanol/ether (10:1:5) gave theanalytical sample, mp 188°-189° C. (dec).

ANALYSIS: Calculated for C₂₁ H₂₄ FN₃ O₂.(CO₂ H)₂ : 60.12% C; 5.70% H;9.15% N. Found: 59.97% C; 5.76% H; 9.08% N.

EXAMPLE 91-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-hydroxyethyl)piperazinedihydrochloride

To 50 ml of dimethylformamide was added 2.6 g of4-(2-hydroxyethyl)piperazine, 6.4 g of3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10.0 g of milledpotassium carbonate, and 0.01 g of potassium iodide. The mixture wasstirred at 90° C. for five hrs, cooled, filtered, and evaporated to anoil. The oil was stirred with 100 ml water for ten mins, and extractedwith ether. The ether extract was washed with water (2×), saturatedsodium chloride solution, and dried over anhydrous magnesium sulfate.After filtering, ethereal hydrogen chloride was added, and theprecipitate was collected and dried to give 4.3 g (57%) of product. Tworecrystallizations from ethyl acetate/methanol, gave the analyticalsample, mp 228° C. (dec).

ANALYSIS: Calculated for C₁₆ H₂₂ FN₃ O₂.2HCl: 50.53% C; 6.36% H; 11.05%N. Found: 50.35% C; 6.44% H; 10.86% N.

EXAMPLE 101-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-pyridyl)piperazinedihydrochloride

To 30 ml of dry dimethylformamide was added 2.45 g of1-(2-pyridyl)piperazine, 3.4 g of3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 8.0 g of sodiumbicarbonate, and a few crystals of potassium iodide. The mixture wasstirred at 100° C. for one and one-half hrs, filtered, the filtrate wasevaporated to an oil. The oil was stirred with 100 ml water for fivemins and extracted with ether. The ether extract was washed with water(2×), saturated sodium chloride and dried over anhydrous magnesiumsulfate. After filtering, the solvent was evaporated to an oil, whichwas dissolved in ether, and converted to a salt by treatment withethereal hydrogen chloride. The salt was recrystallized from ethylacetate/methanol/ether to yield 3.0 g (48%) of product. Tworecrystallizations from ethyl acetate/methanol/ether gave the analyticalsample mp 235°-240° C. (dec).

ANALYSIS: Calculated for C₁₉ H₂₁ FN₄ O.2HCl: 55.21% C; 5.60% H; 13.56%N. Found: 55.56% C; 5.55% H; 13.82% N.

EXAMPLE 11 1-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-methylpiperazinedihydrochloride

To 30 ml of dry dimethylformamide was added 2.0 g of 4-methylpiperazine,4.2 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 8.0 g of sodiumbicarbonate and a few crystals of potassium iodide. After stirring at100° C. for one and one-half hrs, the mixture was filtered. The filtratewas evaporated to an oil. The oil was stirred with 100 ml water for fivemins and extracted with ether. The ether solution was washed with water(2×), saturated sodium chloride solution, dried over anhydrous magnesiumsulfate and filtered. The filtrate was evaporated to an oil, which wasdissolved in ether and converted to a salt by treatment with etherealhydrogen chloride. Recrystallization from ethyl acetate/methanol/ethergave 3.2 g (46%) of product, mp 240° C. (dec). Recrystallization fromethyl acetate/methanol/ether gave the analytical sample, mp 245° (dec).

ANALYSIS: Calculated for C₁₅ H₂₀ FN₃ O.2HCl: 51.43% C; 6.33% H; 12.00%N. Found: 51.26% C; 6.38% H; 11.82% N.

EXAMPLE 121-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-n-propylpiperazinedihydrochloride

A mixture of 8.1 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10g of 1-n-propylpiperazine dihydrobromide, 15 g of potassium carbonateand a few crystals of potassium iodide in 80 ml of dimethylformamide wasstirred at 70° C. for three hrs. The reaction mixture was cooled,filtered and concentrated to an oil, which was stirred with water andextracted with ether. The organic extracts were washed with water (2×),saturated sodium chloride solution, dried over anhydrous magnesiumsulfate, and filtered. The filtrate was treated with ethereal hydrogenchloride to give 10 g (77%) of product, mp 250° C. (dec). The analyticalsample was obtained by recrystallization from ethyl acetate/methanol andhad mp 262° C. (dec).

ANALYSIS: Calculated for C₁₇ H₂₄ FN₃ O.2HCl: 53.97% C; 6.93% H; 11.11%N. Found: 54.28% C; 6.90% H; 11.24% N.

EXAMPLE 131-[3-(6-Flouro-1,2-benzisoxazol-3-yl)propyl]-4-n-butylpiperazinedihydrochloride

A mixture of 12 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10 gof 1-n-butylpiperazine dihydrochloride, 21 g of potassium carbonate anda few crystals of potassium iodide in 80 ml of dimethylformamide wasstirred at 70° C. for three hrs, cooled, filtered and concentrated to anoil. The oil was stirred with water and extracted with ether. The etherextracts were washed with water, saturated sodium chloride solution,dried over anhydrous magnesium sulfate, filtered and concentrated. Theresidue was converted to a salt by treatment with ethereal hydrogenchloride. Recrystallization from ethyl acetate/methanol gave 3.2 g (18%)of product, mp 260° C. (dec).

ANALYSIS: Calculated for C₁₈ H₂₆ FN₃ O.2HCl: 55.10% C; 7.19% H; 10.71%N. Found: 55.20% C; 7.02% H; 10.79% N.

EXAMPLE 141-[3-(6-Fluoro-1,2-benzisoxazol-3-yl)propyl]-4-benzylpiperazinedihydrochloride

A mixture of 13 g of 3-(3-chloropropyl)-6-fluoro-1,2-benzisoxazole, 10 gof 1-benzylpiperazine, 10 g of potassium carbonate and a few crystalspotassium iodide in 80 ml of dimethylformamide, was stirred at ambienttemperature for four hrs and then at 50° C. for one hr. The reactionmixture was cooled, filtered and concentrated to an oil, which wasstirred with water and extracted with ether. The organic extracts werewashed with water (2×), saturated sodium chloride solution, dried overanhydrous magnesium sulfate, filtered and concentrated. The residue wasconverted to 13 g (54%) of product, mp 235°-240° C., by treatment withethereal hydrogen chloride. The analytical sample was obtained byrecrystallization from acetate/methanol.

ANALYSIS: Calculated for C₂₁ H₂₄ FN₃ O.2HCl: 59.16% C; 6.15% H; 9.86% N.Found: 58.90% C; 6.12% H; 9.80% N.

We claim:
 1. A method of treating psychoses comprising administering toa mammal in need of psychoses treatment a psychoses treating effectiveamount of a compound of the formula ##STR7## wherein R is loweralkyl,benzyl, 2-hydroxyethyl, pyridyl or a group of the formula ##STR8##wherein R₁ is hydrogen, loweralkyl, loweralkoxy, halogen ortrifluoromethyl; the optical antipode thereof; or a pharmaceuticallyacceptable acid addition salt thereof.
 2. A method of treating psychosesaccording to claim 1 wherein R is a group of the formula ##STR9##wherein R₁ is hydrogen, loweralkyl, loweralkoxy, halogen ortrifluoromethyl.
 3. A method of treating psychoses according to claim 2wherein R₁ is loweralkoxy.
 4. A method of treating psychoses accordingto claim 3 wherein R₁ is methoxy.
 5. A method of treating psychosesaccording to claim 2 wherein the methoxy group is bound to the2-position of the benzene ring.
 6. A method of treating psychosesaccording to claim 5 wherein the compound is1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-methoxyphenyl)piperazine7. A psychoses treating composition comprising an inert psychosestreating adjuvant and, as the active ingredient, an amount effective intreating psychoses of a compound of the formula ##STR10## wherein R isloweralkyl, benzyl, 2-hydroxyethyl, pyridyl or a group of the formula##STR11## wherein R₁ is hydrogen, loweralkyl, loweralkoxy, halogen ortrifluoromethyl; the optical antipode thereof; or a pharmaceuticallyacceptable acid addition salt thereof.
 8. A psychoses treatingcomposition according to claim 7 wherein R is a group of the formula##STR12## wherein R₁ is hydrogen, loweralkyl, loweralkoxy, halogen ortrifluoromethyl.
 9. A psychoses treating composition according to claim8 wherein R₁ is loweralkoxy.
 10. A psychoses treating compositionaccording to claim 9 wherein R₁ is methoxy.
 11. A psychoses treatingcomposition according to claim 8 wherein the methoxy group is bound tothe 2-position of the benzene ring.
 12. A psychoses treating compositionaccording to claim 11 wherein the compound is1-[3-(6-fluoro-1,2-benzisoxazol-3-yl)propyl]-4-(2-methoxyphenyl)piperazine.